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[目的]探讨熊果酸对人乳腺癌细胞(MCF鄄7)增殖抑制和诱导凋亡的作用,以及凋亡发生与细胞内Ca2+浓度([Ca2+]i)变化的关系。[方法]5～40μmol/L浓度梯度的熊果酸处理MCF鄄7细胞24小时,用MTT比色法测定细胞增殖能力。10μmol/L,20μmol/L和30μmol/L熊果酸处理细胞24小时,用末端脱氧核苷转移酶介导dUTP末端标记法和AnnexinV流式细胞仪法检测凋亡细胞。20μmol/L熊果酸处理细胞24小时,用Fura鄄2荧光负载方法测定[Ca2+]i的变化。[结果]从20μmol/L熊果酸浓度起对MCF鄄7细胞的增殖抑制率显著升高(P<0.01),呈剂量依赖性,半增殖抑制浓度(IC50)为36.18μmol/L。20μmol/L和30μmol/L熊果酸使细胞凋亡率显著升高(P<0.01)。熊果酸处理组的[Ca2+]i明显高于对照组(P<0.05)。[结论]熊果酸具有抑制MCF鄄7细胞增殖和诱导凋亡的作用;其诱导凋亡可能依赖于细胞内Ca2+水平上调。
[Objective] To investigate the effect of ursolic acid on the proliferation and apoptosis induction of human breast cancer cell line MCF-7 and the relationship between apoptosis and the intracellular Ca2 + concentration ([Ca2 +] i). [Method] MCF-7 cells were treated with ursolic acid at a concentration of 5 ~ 40μmol / L for 24 hours. Cell proliferation was measured by MTT assay. The cells were treated with 10μmol / L, 20μmol / L and 30μmol / L ursolic acid for 24 hours. The apoptotic cells were detected by terminal deoxynucleotidyl transferase-mediated dUTP labeling and Annexin V flow cytometry. The cells were treated with 20 umol / L ursolic acid for 24 hours, and the change of [Ca2 +] i was measured by Fura-2 fluorescence loading method. [Result] The inhibitory rate of MCF-7 cells proliferation was significantly increased from 20 umol / L ursolic acid (P <0.01) in a dose-dependent manner. The IC50 was 36.18 μmol / L. The apoptosis rates of 20μmol / L and 30μmol / L ursolic acid increased significantly (P <0.01). The [Ca2 +] i in the ursolic acid treatment group was significantly higher than that in the control group (P <0.05). [Conclusion] UA inhibited the proliferation and induced apoptosis of MCF-7 cells, and its induction of apoptosis may depend on the up-regulation of intracellular Ca2 +.