Vero细胞试验已经被应用在测定人血清中白喉抗毒素的保护力或者通过测定免疫小鼠血清中抗毒素滴度评价白喉类毒素的效力。 白喉毒素能抑制培养物中Vero细胞的新陈代谢和存活,这种毒素效应可以被血清样品中的白喉抗毒素中和。在Vero细胞上,血清样品的滴度在有固定量的白喉毒素存在时被测出。Vero试验是根据在微 Vero cell assays have been used in the determination of diphtheria antitoxin protection in human serum or to evaluate the efficacy of diphtheria toxoid by measuring antitoxin titers in immunized mouse sera. Diphtheria toxin inhibits the metabolism and survival of Vero cells in culture, and this toxin effect can be neutralized by diphtheria antitoxin in serum samples. On Vero cells, serum samples were titrated in the presence of a fixed amount of diphtheria toxin. Vero test is based on micro