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目的用定量RT-PCR法检测“苦参”中抗柯萨奇B病毒有效成份“抗柯注射液”,在Balb/c小鼠病毒性心肌炎模型病毒血症时,对抗“柯萨奇B病毒”的药效。 方法取48只雄性18-22g 8周龄的Balb/c小鼠,随机分成A-H共8组,每组6只。1.各治疗组(A-F组),每只小鼠从腹腔接种0.1ml l04TCID50 CVB3m病毒2h后,每天从尾静脉分别注射抗柯注射液,5、10、15、20、25、30mg/kg,2次/d,连续用药3d。2.病毒组(G组),注射病毒同上法,2h后从尾静脉注入0.3ml无菌生理盐水,2次/d,连续3d。3.空白对照组(H组),腹腔内注射无小牛血清的RPMI-1640培养液,2h后从尾静脉注入0.3ml无菌生理盐水,2次/d,连续3d。 结果 空白组CVB-RNA(-)对接种CVB3m感染后用不同剂量“抗柯注射液”治疗的各小组血液中CVB3m-RNA含量与病毒组相比明显下降,其抑制率(％)与所用抗柯注射液的剂量呈正比,即“抗柯”剂量增加,CVB3m-RNA含量下降的幅度亦增加,每天5-30mg,/kg中的各剂量都有明显抑制病毒增殖的作用。 结论用定量RT-PCR法检测到“抗柯注射液”对Balb/c小鼠病毒性心肌炎病毒血症时效果明显,最小有效剂量为5mg·kg-1·d-1。
OBJECTIVE: To detect “anti-Ke injection”, an effective component of anti-Coxsackie virus B, in Sophora flavescens by quantitative RT-PCR, and to counteract the “Coxsackie B virus” in the viremia of Balb/c mouse viral myocarditis model. “The efficacy of the drug. Methods A total of 48 male 18-22g 8-week-old Balb/c mice were randomly divided into 8 groups of A-H, with 6 mice in each group. 1. In each treatment group (AF group), each mouse was inoculated with 0.1 ml l04TCID50 CVB3m virus from the abdominal cavity for 2 hours, and each day was injected with anti-Ke injection from the tail vein, 5, 10, 15, 20, 25, 30 mg/kg. 2 times / d, continuous medication 3d. 2. In the virus group (G group), the virus was injected in the same manner as the above method. After 2 hours, 0.3 ml of sterile physiological saline was injected from the tail vein twice a day for 3 days. 3. In the control group (H group), RPMI-1640 medium without calf serum was intraperitoneally injected. After 2 hours, 0.3 ml of sterile physiological saline was injected from the tail vein twice a day for 3 days. Results CVB-RNA (-) in blank group significantly decreased the CVB3m-RNA level in blood of each group treated with CVB3m infection with different doses of ”Kangke injection“ compared with the virus group, the inhibition rate (%) and the resistance The dose of Ke injection is proportional, that is, the dose of ”anti-Ke“ increases, and the decrease of CVB3m-RNA content also increases. Each dose of 5-30 mg/kg in each day significantly inhibits the proliferation of the virus. Conclusion Quantitative RT-PCR detection of ”anti-Ke injection" has a significant effect on viral myocarditis viremia in Balb/c mice. The minimum effective dose is 5 mg·kg-1·d-1.