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目的探讨超微铁皮石斛对脾虚便秘小鼠肠道微生物及酶活性的影响。方法采用灌胃番泻叶水煎液7 d后控制饮食,饥饱失常8 d,进行小鼠脾虚便秘造模。设模型组、正常组和治疗组,模型组和正常组灌胃无菌水,治疗组分别灌胃给药铁皮石斛传统汤剂、超微全量汤剂、超微50%量汤剂和超微25%量汤剂,共7 d,然后采集肠道内容物分析肠道菌群及酶活。结果模型组五类可培养微生物(细菌总数、大肠埃希菌、乳酸菌、双歧杆菌和真菌)数量与正常组比较差异均有统计学意义(P<0.01);铁皮石斛传统汤剂组大肠埃希菌数,铁皮石斛超微50%量汤剂组细菌总数、大肠埃希菌和真菌数,超微25%量汤剂组细菌总数和大肠埃希菌数均达到正常组水平(P>0.05);铁皮石斛超微全量汤剂组和超微50%量汤剂组对纤维素酶的影响作用相当,均达到正常组水平(P>0.05),铁皮石斛三种不同剂量的超微汤剂对木聚糖酶活性的影响作用相当(P>0.05),铁皮石斛超微全量汤剂组淀粉酶活性与正常组相当(P>0.05)。结论铁皮石斛传统汤剂、超微全量汤剂和超微50%量汤剂对脾虚便秘均有很好的疗效,能够调控肠道微生态平衡及肠道酶活性。
Objective To investigate the effects of Dendrobium candidum on gut microbiota and enzyme activity in mice with spleen deficiency constipation. Methods The rats were fed with the decoction of senna decoction for 7 days to control the diet, the starvation was abnormal for 8 days, and the models of spleen deficiency constipation were established. The model group, the normal group and the treatment group, the model group and the normal group were given gavage with sterile water. The treatment group were given gavage of traditional decoction of Dendrobium officinale, superfine total decoction, super 50% decoction and ultramicro 25% amount of decoction, a total of 7 d, and then collected intestinal contents of intestinal flora and enzyme activity. Results There were significant differences among the five groups of culturable microorganisms (total number of bacteria, Escherichia coli, Lactobacillus, Bifidobacterium and fungi) in the model group compared with the normal group (P <0.01) The number of bacteria, the number of bacteria, the number of Escherichia coli and fungi, the number of bacteria and the number of Escherichia coli in the ultrafine 25% decoction group reached the normal level (P> 0.05 ); Dendrobium officinale superfine full decoction group and superfine 50% decoction group had the same effect on cellulase, both reached the normal group level (P> 0.05), Dendrobium candidum three different doses of super-decoction (P> 0.05). The amylase activity in Dendrobium officinale supernatant group was similar to that in normal group (P> 0.05). Conclusion Traditional decoction of Dendrobium candidum, superfine total decoction and superfine 50% decoction have good curative effect on constipation of spleen deficiency, and can regulate intestinal microflora balance and intestinal enzyme activity.