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目的： 采用反相ＨＰＬＣ 法， 分离测定复方赖氨酸颗粒中的盐酸赖氨酸。方法： 用２ ， ４ － 二硝基氟苯为衍生化试剂， 将盐酸赖氨酸进行衍生化。以十八烷基硅烷键合硅胶为填充剂， 甲醇－０－０５ ｍｏｌ·Ｌ－ １ 醋酸钠和醋酸溶液（６５∶３５） 为流动相， 检测波长为３６０ ｎｍ 。结果： 盐酸赖氨酸浓度在２－７ ～２７ μｇ·ｍ Ｌ－ １ 范围内线性关系良好， ｒ ＝ ０－９９９ ９ ， 回归方程 Ｃ＝ ２－５９１ ×１０ － ５ Ａ－ ０－３１６ ６ 。平均回收率为１００－２ ％ ， ＲＳＤ 为０－６６ ％ （ ｎ ＝ ６） 。结论： 本法可用于测定复方赖氨酸颗粒中盐酸赖氨酸含量。
Objective: To isolate and determine lysine hydrochloride in compound lysine granules by reverse phase HPLC. Methods: Lysine hydrochloride was derivatized with 2, 4 - dinitrofluorobenzene as derivatization reagent. Using octadecylsilane bonded silica as the filler, methanol -0-05 mol·L -1 sodium acetate and acetic acid (65:35) as the mobile phase, the detection wavelength was 360 nm. Results: The linearity of lysine hydrochloride in the range of 2-7-27 μg · m L -1 was good, r = 0-999 9, regression equation C = 2-591 × 10-5 A-0-316 6. The average recovery was 100-2% and RSD was 0-66% (n = 6). Conclusion: This method can be used to determine the content of lysine hydrochloride in compound lysine granules.