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目的 :探讨检测AML1 ETO融合转录本在t( 8;2 1)急性粒细胞白血病M 2型 [M 2 /t( 8;2 1]的临床诊断和预后判断中的意义。方法 :用半筑巢式逆转录聚合酶链反应 (RT/PCR)技术检测AML1/ETO融合基因转录本 ,应用Southernblot技术及限制性内切酶技术检测PCR产物的特异性。结果 :在 19例AML M 2中共检出 10例存在AML1/ETO融合基因转录本 ,阳性率为 5 2 .6 % ,2例MDS中 1例为阳性 ,其余病例包括 3例M4均为阴性 ,第一轮PCR的检测水平为 1/ 10 3 10 4 个细胞 ,第二轮PCR的敏感性达 1/ 10 4 10 5个细胞 ,说明该技术的敏感性很高。结论 :半筑巢式RT/PCR技术是一种快速、简便、灵敏、可靠的检测AML1/ETO融合转录本的方法 ,对M2 /t( 8;2 1)的诊断及预后判断具有重要临床价值
Objective: To explore the significance of detecting AML1 ETO fusion transcripts in the clinical diagnosis and prognosis of acute myelogenous leukemia M 2 [M 2 /t ( 8 ;21]). Methods: Nested reverse transcriptase polymerase chain reaction (RT/PCR) was used to detect AML1/ETO fusion gene transcripts, and the specificity of the PCR products was detected by Southern blot technique and restriction endonuclease technique.Results: A total of 19 cases of AML M 2 were co-detected. Out of the 10 cases, AML1/ETO fusion gene transcripts were present, with a positive rate of 52.6%. One case was positive in 2 cases of MDS. The remaining cases including 3 cases were negative in M4. The first round of PCR detection was 1/1. 10 3 10 4 cells, the sensitivity of the second round of PCR reached 1/10 4 10 5 cells, indicating that the technique has high sensitivity.Conclusion: Semi-nested RT/PCR technology is a fast, simple, Sensitive and reliable detection of AML1/ETO fusion transcripts has important clinical value in the diagnosis and prognosis of M2/t( 8;21)