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目的:探讨高糖状态下肌肌醇加氧酶(MIOX)表达变化对肾小管上皮细胞TGF-β1表达的影响。方法:①在体外培养大鼠肾小管上皮细胞NRK-52E,应用不同浓度高糖刺激NRK-52E细胞48h,观察MIOX、TGF-β1 mRNA和蛋白表达的改变;②观测不同浓度TGF-β1刺激NRK-52E细胞48h后MIOX mRNA和蛋白表达的变化;③应用针对MIOX基因的ASODN干涉NRK-52E细胞抑制MIOX基因表达后再用高糖刺激,观测其对TGF-β1 mRNA和蛋白表达的影响。结果:①不同浓度(15、30mmol/L)高糖刺激NRK-52E细胞48h后MIOX、TGF-β1 mRNA和蛋白表达增加;②不同浓度(1、5、10、25mmol/L)TGF-β1刺激NRK-52E细胞48h后MIOX mRNA和蛋白表达无明显变化;③针对MIOX基因的ASODN干涉NRK-52E细胞使MIOX基因表达下降,同时NRK-52E细胞TGF-β1 mRNA和蛋白的表达也下降。结论:本研究结果提示,高糖刺激肾小管上皮细胞能使MIOX、TGF-β1 mRNA和蛋白表达增加,而且MIOX表达异常可能通过影响细胞因子TGF-β1的表达在糖尿病肾病中起作用。
Objective: To investigate the effect of myo-inositol oxygenase (MIOX) expression on the expression of TGF-β1 in renal tubular epithelial cells under high glucose. Methods: ①The NRK-52E cells were cultured in vitro and NRK-52E cells were treated with different concentrations of high glucose for 48 hours to observe the changes of MIOX and TGF-β1 mRNA and protein expression. ② The effects of different concentrations of TGF-β1 on NRK- -52E cells 48h after MIOX treatment; ③The effect of ASODN on MIOX gene expression was observed after NRK-52E cells were inhibited by high glucose and MIOX gene expression. RESULTS: ① The mRNA and protein expressions of MIOX and TGF-β1 were increased after NRK-52E cells were treated with different concentrations of 15 and 30mmol / L for 48h; ② The stimulation of TGF-β1 The expression of MIOX mRNA and protein in NRK-52E cells did not change significantly after 48h; ③ The expression of MIOX gene in NRK-52E cells induced by ASODN against MIOX gene decreased, while the expression of TGF-β1 mRNA and protein in NRK-52E cells also decreased. CONCLUSION: The results of this study suggest that high glucose can stimulate the expression of MIOX and TGF-β1 mRNA and protein in renal tubular epithelial cells, and the abnormal expression of MIOX may play a role in diabetic nephropathy by affecting the expression of TGF-β1.