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在光学显微镜下观察和测定了己酮可可碱(PF)和2-脱氧腺苷(2-DA)在体外对冷冻复温后精液精子的活率、活力及其毛细管穿透值的影响。结果显示PF和2-DA均能提高冷冻复温后精子的活率、活力及其毛细管穿透值,其最适浓度分别为4.0mmol/L和2.0mmol/L,与对照组比较均有显著性差异(P<0.01)。两级间比较,2-DA优于PF(P<0.01)。提示PF与2-DA合用不具有协同作用,反而有相互抑制作用,认为单独使用2-DA比合用PF效果更好。
The effects of pentoxifylline (PF) and 2-deoxyadenosine (2-DA) on the sperm motility and capillary permeability after freezing and rewarming were observed under a light microscope. The results showed that both PF and 2-DA increased sperm motility and capillary permeability after freezing and thawing, with the optimal concentration of 4.0 mmol / L and 2.0 mmol / L, respectively. Compared with the control group There was significant difference (P <0.01). 2-DA was better than PF (P <0.01). Tip PF and 2-DA combination does not have a synergistic effect, but have mutual inhibitory effect, that the use of 2-DA alone than with PF better.