目的 观察特异性脱颗粒肥大细胞的形态特征。方法 通过抗ＩｇＥ单抗（抗ＩｇＥ ｍＡｂ，ＨＲＰ）与已致敏的肥大细胞相结合，用ＤＡＢ加中胜红双染色法对肥大细胞进行染色。结果 发现无论肥大细胞是否脱颗粒，只有被ＩｇＥ致敏的肥大细胞才能被染成棕褐色；而非特异性（即未被ＩｇＥ致敏的肥大细胞），无论其脱颗粒与否，都呈现中性红的颜色，即胞浆内呈现砖红色的颗粒。结论 该实验为评价患者当前所处状态（是高敏体质还是非高敏体质？是疾病缓解期抑或发作期等？）、预测哮喘等Ⅰ型变态反应的病程转归、判断疗效，以及进行一些与肥大细胞脱颗粒有关的科学实验，提供了一种新的更客观的方法。 Objective To observe the morphological characteristics of specific degranulation mast cells. Methods Mast cells were stained with DAB plus MRS by anti-IgE mAb (HRP) and sensitized mast cells. Only mast cells that were sensitized with IgE were found to be septa, irrespective of mast cells degranulation, whereas nonspecific (ie, mast cells that were not sensitized by IgE) showed neutrality regardless of degranulation Red color, that is, cytoplasm brick red particles. Conclusions This experiment is to evaluate the current course of the patient (whether it is hypersensitivity or non-hypersensitivity? Is the disease remission period or episode, etc.), to predict the course of the type I allergic reaction such as asthma, to determine the therapeutic effect, Scientific experiments related to cell degranulation provide a new, more objective approach.