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检测长期培养启动细胞 (LTC IC)含量可反映造血干细胞的存在和变化。用正常人骨髓基质细胞、人骨髓基质细胞系和转基因骨髓基质细胞系做培养底层 ,接种CD34+ 细胞 ,进行连续 8周的长期体外培养。研究结果表明 ,在共转染FL和TPO基因的基质细胞系HFT为培养底层所产生的LTC IC为 3.6 5± 0 .5 8 10 0 0CD34+ 细胞 ,在原代骨髓基质细胞层上所产生的LTC IC为2 .6 5± 0 .76 10 0 0CD34+ 细胞 ,二者在统计学上无显著性意义。结论 :转染FL TPO基因的HFT细胞系可作为LTC IC培养的培养底层细胞
Detection of long-term cultured starter cells (LTC IC) content can reflect the presence and changes of hematopoietic stem cells. Using normal human bone marrow stromal cells, human bone marrow stromal cell line and transgenic bone marrow stromal cell line as culture bottom, CD34 + cells were seeded and cultured for a long time in vitro for 8 weeks. The results showed that the LTC IC produced by the substrate cell line HFT co-transfected with the FL and TPO genes was 3.6 5 ± 0.58 10 0 0 CD34 + cells and the LTC IC produced on the primary bone marrow stromal cell layer Was 2. 6 5 ± 0 .76 10 0 0 CD34 + cells, both of which were not statistically significant. CONCLUSION: The HFT cell line transfected with the FL TPO gene can be used as a culture basal cell for LTC IC culture