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用 10 0条 10碱基随机引物 ,以普通小麦中国春、中间偃麦草为材料进行 RAPD分析 ,筛选到一个偃麦草染色体组特异引物 OPF0 3,并从中间偃麦草中克隆了该引物的特异 DNA片段 OPF0 312 91。将该片断与比萨偃麦草中的 OPF0 312 96(Gen Bank序号 U4 35 16 )比较 ,同源性为 88%。根据 OPF0 312 91的序列 ,设计了 2对SCAR引物 ,利用 OPF0 3和引物 P3、P4对普通小麦、普通小麦 -中间偃麦草的部分双二倍体、长穗偃麦草、中间偃麦草、小麦 -二倍体长穗偃麦草代换系、附加系共 6类材料进行了 RAPD和 SCAR分析 ,发现 RAPD标记OPF0 312 91没有出现在含 Ee染色体组的材料中 ,而标记 SCAR982 则出现于所有含 E染色体组的材料中。说明 ,根据 Eb 染色体组特异 RAPD标记转化的 SCAR标记 ,可以同时检测小麦背景下的 Ee 染色体
A 10-bp random primer was used to analyze the RAPD analysis of common wheat Chinese Spring and Thinopyrum intermedium. A primer of chromosome 3 (OPF0 3) was screened and the specific DNA of this primer was cloned Fragment OPF0 312 91. The fragment was 88% homologous to OPF0 312 96 (GenBank No. U4 35 16) in Thinopyrum. According to the sequence of OPF0 312 91, two pairs of SCAR primers were designed, using OPF0 3 and primers P3 and P4 for partial diploids of common wheat, common wheat-Thinopyrum intermedium, The results showed that the RAPD marker OPF0 312 91 did not appear in the Ee chromosome-containing material, while the marker SCAR982 appeared in all the E In the genome of the material. This shows that the Ee chromosomes in the wheat background can be detected simultaneously based on the SCAR markers transformed by the Eb genome-specific RAPD markers