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目的 探讨不同胃粘膜病变端粒状态的变化规律。方法 采用Ｓｏｕｔｈｅｒｎ 杂交技术，检测３５ 例胃癌及相应的３３ 例癌旁和１９ 例手术切缘组织端粒限制性片段长度（ｔｅｌｏｍｅｒｉｃ ｒｅｓｔｒｉｃｔｉｏｎ ｆｒａｇｍｅｎｔＴＲＦ） 。结果 与癌旁和／或手术切缘组织比较，在３５ 例肿瘤组织中，ＴＲＦ缩短、无变化及延长分别占２０（５７．１％ ）、１２（３４．３ ％） 及３（７ ．６％ ） 例；进一步研究发现，由正常胃粘膜（Ｎ） →慢性萎缩性胃炎（ＣＡＧ）→肠上皮化生（ＩＭ）→异型增生（Ｄｙｓ） →胃癌（ＧＣ） 的发展过程中，ＴＲＦ有逐渐缩短的趋势，且ＧＣ 组ＴＲＦ长度明显短于Ｎ 及ＣＡＧ 组（ Ｐ＜ ０ ．０１） ；肿瘤组织ＴＲＦ与患者性别、肿瘤大小、分化程度、临床分期及端粒酶活性大小等临床病理指标无明显相关性，而与患者年龄有一定的关系。结论 由于受多种因素的影响，与肿瘤组织中端粒酶阳性率相比较，端粒限制性片段长度可能不宜作为衡量肿瘤生物学行为的一个良好的指标。
Objective To investigate the changes of telomere status in different gastric mucosal lesions. Methods Southern hybridization technique was used to detect telomeric restriction fragment (TRF) in 35 cases of gastric cancer and corresponding 33 cases of paraneoplastic and 19 surgical margins. Results Compared with para-carcinoma and/or surgical margin tissue, TRF shortened, no change, and prolonged tumors accounted for 20 (57.1%), 12 (34.3%), and 3 (3.6%) in 35 tumor tissues. %) A further study found that TRF gradually develops from normal gastric mucosa (N) to chronic atrophic gastritis (CAG) → intestinal metaplasia (IM) → dysplasia (Dys) → gastric cancer (GC) The shortened trend was that the TRF length in the GC group was significantly shorter than that in the N and CAG groups (P < 0.01). The clinicopathological parameters of tumor tissue TRF and patient gender, tumor size, differentiation, clinical stage, and telomerase activity were not Obvious correlation, but with the patient's age has a certain relationship. Conclusion Due to a variety of factors, compared with the positive rate of telomerase in tumor tissues, the length of telomere restriction fragments may not be a good indicator to measure the biological behavior of tumors.