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目的了解甘肃省会宁县兔弓形虫的感染情况。方法酚试剂方法提取家兔血液中总DNA,将弓形虫529 bp多拷贝片段基因作为待检弓形虫序列,通过单管一步法巢式PCR技术检测兔弓形虫感染情况。结果共检测117份兔血液样本,弓形虫阳性13份,阳性率为11.11%(13/117)。散养兔(在兔圈中散养)阳性率为11.63%(5/43),笼养兔(单只笼养)阳性率为10.81%(8/74),差异无统计学意义(P>0.05);雌兔阳性率为8.70%(6/69),雄兔阳性率为14.58%(7/48),差异无统计学意义(P>0.05);獭兔阳性率为11.39%(9/79),长耳兔阳性率为10.35%(4/38),差异无统计学意义(P>0.05)。结论甘肃省会宁县农村兔弓形虫感染较为普遍,其感染率与兔的饲养方式、雌雄以及品种无关。
Objective To understand the infection status of Toxoplasma gondii in Huining County, Gansu Province. Methods Phenol reagent method was used to extract the total DNA of rabbits blood. Toxoplasma gondii 529 bp multicopy fragment gene was used as Toxoplasma gondii to be tested. Results A total of 117 rabbit blood samples were detected. Toxoplasma positive 13, the positive rate was 11.11% (13/117). The positive rate of free rabbits in the rabbit circle was 11.63% (5/43), and that in the cage rabbits (single cage) was 10.81% (8/74), with no significant difference (P> 0.05). The positive rate of rabbits was 8.70% (6/69) and the positive rate of male rabbits was 14.58% (7/48) (P> 0.05). The positive rate of rex rabbits was 11.39% (9/79) ), The positive rate of long-eared rabbits was 10.35% (4/38), the difference was not statistically significant (P> 0.05). Conclusion Toxoplasma gondii infection is more common in rural Huining County, Gansu Province. The infection rate has nothing to do with the breeding methods, male and female, and breed of rabbits.