Objective To develop a simple and fast method for removing polyethylene glycol(PEG) and simultaneous determination of fives saponins, i.e. astragaloside IV, notoginsenoside R_1, ginsenoside Rg_1, ginsenoside Rb_1, and ginsenoside Rd in dripping pills made from Astragali Radix and Panax notoginseng. Methods The extraction method was based on a liquid-liquid extraction using water-saturated n-butanol and the quantitative determination was based on ultra-performance liquid chromatography coupled with evaporative light scattering detection(UPLC-ELSD). The chromatographic analysis was performed on an Acquity UPLC HSS T3 column(100 mm × 2.1 mm, 1.8 μm) with a gradient elution of acetonitrile-0.1% formic acid aqueous solution within a runtime of 15 min. Results Compared to different methods, the proposed method could remove the interference of PEG in formulation. And the calibration curves showed good linearity during the test ranges. The method was validated for limits of detection and quantification, precision, and reproducibility. The recoveries were within the range of 96.87%-99.97%. In addition, the verified method was firstly applied to determination of the five active ingredients in Qishen Yiqi Dripping Pills(QYDP) simultaneously.Conclusion The contents of five active ingredients are stable and homogeneous in QYDP, which indicates that the method could be readily utilized as a quality evaluation method for this traditional Chinese medicine dripping pills made from Astragali Radix and Panax notoginseng. Objective To develop a simple and fast method for removing polyethylene glycol (PEG) and simultaneous determination of fives saponins, ie astragaloside IV, notoginsenoside R_1, ginsenoside Rg_1, ginsenoside Rb_1, and ginsenoside Rd in dripping pills made from Astragali Radix and Panax notoginseng. Methods The extraction method was based on a liquid-liquid extraction using water-saturated n-butanol and the quantitative determination was based on ultra-performance liquid chromatography coupled with evaporative light scattering detection (UPLC-ELSD). The chromatographic analysis was performed on an Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm) with a gradient elution of acetonitrile-0.1% formic acid aqueous solution within a runtime of 15 min. Results Compared to different methods, the proposed method could remove the interference of PEG in And the calibration curves showed good linearity during the test ranges. The method was validated for limits of detection and qua ntification, precision, and reproducibility. The recoveries were within the range of 96.87% -99.97%. In addition, the verified method was first applied to determination of the five active ingredients in Qishen Yiqi Dripping Pills (QYDP) simultaneously. Conclusions The contents of five active ingredients are stable and homogeneous in QYDP, which indicates that the method could be readily utilized as a quality evaluation method for this traditional Chinese medicine dripping pills made from Astragali Radix and Panax notoginseng.