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DNA芯片是一种基因检测的新技术。采用瓷片材料 ,代替常用的硅或玻片等材料 ,制备DNA微集芯片 ,研究了该方法的可行性和特异性 ,并讨论了该方法的优越性。通过自制的DNA微集芯片打印装置 ,将噬菌体DNA和肌动蛋白基因的探针打印成 2 0× 2 0的致密点阵 ,密度为每平方厘米 80 0点阵。结果 :采用瓷片打印的芯片 ,可以耐受至少 5次分子杂交过程 ,未见明显探针点阵DNA的减少 ,且DNA杂交的特异性较高。采用该方法 ,可制备具有较高特异性且可多次使用的DNA微集芯片。
DNA chip is a new technology for genetic testing. The porcelain chip material was used instead of the commonly used materials such as silicon or glass slides to prepare DNA microarray chips. The feasibility and specificity of the method were studied. The superiority of the method was also discussed. Probes of bacteriophage DNA and actin genes were printed into 20 × 20 dense lattice arrays with a density of 80 0 lattices per square centimeter through a homemade DNA microarray printing device. Results: The chip printed with ceramic chips can tolerate at least 5 times molecular hybridization, no obvious decrease of probe DNA, and high specificity of DNA hybridization. With this method, a DNA microchips chip with high specificity and reusability can be prepared.