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喀西茄(Solanum khasianum)由于含有生成甾类激素的基础物质而已经受到重视。它不但含有甾体生物碱甙,例如solamargrine和solasonine和khasianine,还含有丰富的甾体皂甙。本次报道了从喀西茄果实中分离得到的一种新的甾体皂甙——solakhasoside(1)。 实验方法:喀西茄干果实(来源于日本九州大学)用甲醇提取,提取液浓缩,残留物用MCI柱层析分离,以40％～100％的甲醇为梯度洗脱剂,60％甲醇洗脱部分再经过Cosmosil 75 C_(18)-OPN(40％～70％甲醇为洗脱剂)和硅胶柱层析分离(以氯
Solanum khasianum has received attention because it contains basic substances that produce steroid hormones. It not only contains steroidal alkaloids such as solamargrine and solansonine and khasianine, but it is also rich in steroidal saponins. A new steroidal saponin, solakhasoside (1), was isolated from the fruit of Kasiti. Experimental method: Caragana stem fruits (from Kyushu University, Japan) were extracted with methanol and the extracts were concentrated. The residue was separated by MCI column chromatography and eluted with a 40% to 100% methanol gradient, and 60% methanol washes. The desorbed fractions were separated by silica gel column chromatography using Cosmosil 75 C_(18)-OPN (40% to 70% methanol as eluent).