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目的优化氧化分解白术挥发油(decomposed volatile oil from Atractylodes macrocephala,DVOA)与羟丙基-β-环糊精(HP-β-CD)的包合工艺。方法采用冷冻干燥法制备包合物,荧光分光光度法评价制备工艺。以包合率和含油率为指标,采用正交试验法,考察HP-β-CD与DVOA质量比、包合温度和包合时间对包合的影响,筛选出最佳包合工艺条件。通过紫外-可见光谱(UV-vis)、荧光光谱(FL)和显微成像法表征包合物,并进行稳定性测试,同时利用气相色谱-质谱联用(GC-MS)技术对DVOA包合前后的化学成分进行分析。结果 DVOA与HP-β-CD形成了包合物,其最佳包合条件为HP-β-CD与DVOA质量比为10:1,包合温度30℃,包合时间2.0 h,平均包合率和含油率分别为73.32%和10.43%。包合前后DVOA的化学成分相同,包合过程只是对各组分的比例略有影响。结论以最佳工艺条件制备的DVOA-HP-β-CD包合物工艺合理、稳定可行,可为DVOA的抗肿瘤新药研发提供可靠的实验基础。
Objective To optimize the inclusion process of decomposed volatile oil from Atractylodes macrocephala (DVOA) and hydroxypropyl-β-cyclodextrin (HP-β-CD). Methods The inclusion complex was prepared by freeze-drying method and its preparation was evaluated by fluorescence spectrophotometry. Taking the inclusion rate and oil content as indexes, the orthogonal test was used to investigate the influence of the mass ratio of HP-β-CD to DVOA, the inclusion temperature and the inclusion time on the inclusion, and the optimum inclusion conditions were screened out. The inclusion complex was characterized by UV-vis spectroscopy, fluorescence spectroscopy (FL) and microscopic imaging, and its stability was tested. Meanwhile, the inclusion of DVOA by gas chromatography-mass spectrometry (GC-MS) Before and after the chemical composition analysis. Results The inclusion complex was formed between DVOA and HP-β-CD. The optimal inclusion conditions were as follows: the mass ratio of HP-β-CD to DVOA was 10: 1, the inclusion temperature was 30 ℃, the inclusion time was 2.0 h, Rate and oil content were 73.32% and 10.43% respectively. The chemical composition of DVOA before and after inclusion is the same, and the inclusion process only slightly affects the proportion of each component. Conclusion The DVOA-HP-β-CD inclusion complex prepared under the optimal conditions is reasonable, stable and feasible, which can provide a reliable experimental basis for the development of new antitumor drugs of DVOA.