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目的应用荧光金(FG)、快蓝(FB)、辣根过氧化物酶(HRP)逆行神经追踪技术研究盆神经节(MPG)、尿道外括约肌(EUS)与骶髓排尿中枢之间的神经通路。方法选用正常雄性SD大鼠,盆神经节、尿道外括约肌、尿道黏膜下、膀胱逼尿肌内注射神经追踪剂组织化学反应及荧光显微镜。结果标记神经元在脊髓L6部至S1侧角(以L6多见)可见FB阳性神经元,L6脊髓前角的腹外侧部,即Onuf’s核,同时中间外侧柱(IML)、中央管周围出现FG阳性神经元。盆神经节在荧光显微镜下可发现HRP暗黑色阳性神经细胞和呈蓝色的荧光阳性神经细胞;脊髓L6部至S1灰质后联合核、中央管周围I、ML区同时出现FG、FB细胞,但未发现FG、FB的双标细胞。结论大鼠脊髓内排尿中枢主要位于脊髓L6-S1节段,主要包括支配盆神经节的PGN和支配EUS的前角神经元。灰质后联合核、中央管周围I、ML区内的亦可见少许神经元。盆神经节支配膀胱逼尿肌,是排尿反射的最后一级神经元。
Objective To study the relationship between nerve root ganglion (MPG), urethral sphincter (EUS) and sacral cord urinary center using fluorescent gold (FG), fast blue (FB) and horseradish peroxidase (HRP) path. Methods Normal male SD rats, pelvic ganglia, external urethral sphincter, submucosal urethra and bladder detrusor were injected with neurotrophic agent histochemical reaction and fluorescence microscope. RESULTS: FB positive neurons were seen in the L6 to S1 side of the spinal cord (more common in L6). The ventral part of L6 anterior horn of the spinal cord, ie Onuf’s nucleus, while the medial lateral column (IML) appeared around the central canal Positive neurons. In the pelvic ganglia, HRP dark positive neurons and blue positive neurons were found under the fluorescence microscope. The nuclei of the spinal cord from L6 to S1 gray matter of the spinal cord combined with the appearance of FG and FB cells in I and ML around the central canal Did not find FG, FB double-labeled cells. Conclusion The urinary centralis of rat spinal cord is mainly located in the L6-S1 segment of the spinal cord, mainly including PGNs that dominate the pelvic ganglia and anterior horn neurons that dominate the EUS. After the combination of gray matter nuclear, central canal around I, ML area can also be seen in a small number of neurons. The pelvic ganglia dominate the detrusor of the bladder and are the last neurons of the urination reflex.