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本项研究将乙炔和氯霉素抑制技术结合起来 ,对丹麦一森林土壤的反硝化作用进行了研究 ,并考察温度对其还原酶活性的影响 .反硝化还原酶活性和合成过程受O2 的抑制 ,厌氧培养时 ,需要一定时间消耗系统中残余的O2 来解除这种抑制作用 .在无抗生素抑制蛋白质合成时 ,硝酸还原酶只有少量合成 ,而N2 O还原酶却显著地诱导产生 .这一结果对土壤吸收N2 O能力的研究具有重要意义 .在各处理下 ,系统中未发生亚硝酸盐的明显积累 ,表明亚硝酸还原酶活性大于硝酸还原酶 .外加葡萄糖加速了反硝化作用 ,并能促进酶的合成和消除还原过程中的电子竞争 .供试土壤表现出很强的厌氧呼吸作用 ,并受外加C源的促进 .反硝化作用的活化能低于土壤厌氧呼吸的活化能 ,因此反硝化作用的Q1 0值较低 ,CO2 和N2 O的产生比例随温度升高而加大 .
In this study, the technology of acetylene and chloramphenicol inhibition was combined to study the denitrification of a Danish soil and to investigate the effect of temperature on its reductase activity. The denitrification reductase activity and the synthesis process were inhibited by O2 , Anaerobic culture, it takes some time to consume residual O2 in the system to lift this inhibition.In the absence of antibiotics to inhibit protein synthesis, nitrate reductase only a small amount of synthesis, and N2O reductase was induced significantly. The results of the study on the ability of soil to absorb N2 O are of great significance.Under the treatments, no significant accumulation of nitrite was observed in the system, indicating that nitrite reductase activity was greater than that of nitrate reductase.Addition of glucose accelerated denitrification and Promote the synthesis of the enzyme and eliminate the electronic competition in the reduction process.The test soil showed a strong anaerobic respiration and was accelerated by the addition of C. The activation energy of the denitrification was lower than that of the soil anaerobic respiration, As a result, the value of Q 1 0 for denitrification is lower and the proportion of CO 2 and N 2 O increases with increasing temperature.