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鼻咽癌(nasopharyngeal carcinoma,NPC)在头颈部肿瘤中位居首位,对人们的身体健康构成严重威胁。放射治疗是目前治疗鼻咽癌的有效手段,但是鼻咽癌组织存在对放射射线不敏感的细胞,这对放疗敏感性提出了更高要求。为探究miR-421下调FOXO4对鼻咽癌细胞CNE-2放疗敏感性的作用,本研究先对miR-421靶基因进行预测,然后考察不同放疗剂量对miR-421和FOXO4 mRNA表达水平的影响,直接证明miR-421及FOXO4与鼻咽癌细胞放疗敏感性有关,最后通过存活曲线、流式细胞仪检测、MTT法,探究了miR-421上调、FOXO4沉默对鼻咽癌细胞CNE-2放疗敏感性的影响。结果发现,FOXO4为miR-421的潜在靶基因,鼻咽癌细胞CNE-2经放射处理后,miR-421的表达水平明显下降,FOXO4的表达水平显著升高;miR-421上调和FOXO4沉默均增强了鼻咽癌细胞放疗的敏感性,降低了癌细胞存活率和克隆形成率,提高了的癌细胞凋亡率。可得知,miR-421通过负调控FOXO4靶基因增强鼻咽癌细胞CNE-2放疗的敏感性,这为进一步深入研究miR-421对其靶基因FOXO4的作用机制打下基础。
Nasopharyngeal carcinoma (NPC) ranks first in the head and neck cancer and poses a serious threat to people’s health. Radiation therapy is an effective method for the treatment of nasopharyngeal carcinoma. However, there are cells that are not sensitive to radiation in nasopharyngeal carcinoma, which puts forward higher requirements on radiosensitivity. In order to investigate the role of miR-421 in down-regulating the sensitivity of FOXO4 to nasopharyngeal carcinoma cell line CNE-2, we first predicted miR-421 target gene and then investigated the effect of different doses of radiotherapy on miR-421 and FOXO4 mRNA expression. Directly demonstrate the miR-421 and FOXO4 nasopharyngeal cancer cell radiosensitivity, and finally by survival curve, flow cytometry, MTT assay to explore the miR-421 upregulation, FOXO4 silencing CNE-2 nasopharyngeal carcinoma cell radiation sensitive Sexual effects. The results showed that FOXO4 was a potential target gene of miR-421. The expression of miR-421 was significantly decreased and the expression of FOXO4 was significantly increased after CNE-2 treatment of nasopharyngeal carcinoma cells. Upregulation of miR-421 and FOXO4 silencing Enhance the sensitivity of radiotherapy of nasopharyngeal carcinoma cells, reduce the survival rate of cancer cells and clone formation rate, and improve the apoptosis rate of cancer cells. It can be learned that miR-421 negatively regulates the sensitivity of FOXO4 target gene to CNE-2 radiotherapy in nasopharyngeal carcinoma cells, which lays the foundation for further study on the mechanism of miR-421 on its target gene FOXO4.