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通过对１９对生育夫妇和７２对不育夫妇丈夫精液中绿色吖淀橙（ＡＯ）荧光精子（成熟精子）比率分布情况的比较分析，探讨了绿色ＡＯ荧光精子百分率与精液参数及精子授精能力之间的关系。在１～３年随访中，通过人工授精、其他治疗或在非治疗期，５４对绿色ＡＯ荧光精子≥５０％的夫妇有１８对怀孕，而１８对绿色ＡＯ荧光精子＜５０％的夫妇无１例怀孕。结果还显示了绿色ＡＯ荧光精子百分率与精液参数无关。认为该项指标从精子核ＤＮＡ成熟状态角度反映了单凭精液分析所不能反映的男性生育力。精液中绿色ＡＯ荧光精子＜５０％，是低生育力精液的评估界限
Through the comparative analysis of the ratios of green acridine orange (AO) fluorescent sperm (mature sperm) in the semen of 19 pairs of reproductive couples and 72 pairs of sterile couples, the relationship between the percentage of green AO fluorescence sperm and sperm parameters and sperm fertilization Relationship between. Among the 1 to 3 years of follow-up, there were 18 pairs of pregnancies by artificial insemination, other treatment or during non-treatment, 54 pairs of couples with ≥50% green AO fluorescence sperm, and 18% of couples with less than 50% of green AO fluorescence sperm Cases of pregnancy. The results also show that the percentage of green AO-fluorescent sperm is unrelated to semen parameters. It is considered that this index reflects male fertility, which can not be reflected by semen analysis alone, from the perspective of maturity of sperm DNA. Semen in the green AO fluorescence sperm <50%, is the assessment of low fertility semen