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目的:建立高效液相色谱法同时测定加味香连丸中木香烃内酯和去氢木香内酯含量。方法:采用Waters SunFire C18(4.6 mm×150 mm,5μm)色谱柱,流动相为乙腈-水(50:50,v/v),流速1.0 mL.min-1,检测波长225 nm,柱温为室温。结果:木香烃内酯和去氢木香内酯的浓度分别在2.65～26.52μg.mL-1(r=0.9998)和2.51～25.07μg.mL-1(r=0.9998)范围内与峰面积呈良好的线性关系;平均回收率(n=9)分别为99.7%和100.0%。结论:本方法快速简便,重复性好,专属性强,可作为加味香连丸的质量控制方法之一。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of androstanediol in Jiawei Xianglian pills. METHODS: Waters SunFire C18 (4.6 mm × 150 mm, 5 μm) was used. The mobile phase was acetonitrile-water (50:50 v / v) at a flow rate of 1.0 mL.min-1. Room temperature. Results: The results showed that the concentrations of the wood-borneolide and dehydrocostusol were in the range of 2.65-26.52μg.mL-1 (r = 0.9998) and 2.51-25.07μg.mL-1 (r = 0.9998) Showed a good linear relationship; average recovery (n = 9) were 99.7% and 100.0%. Conclusion: The method is rapid, simple, reproducible and specific. It can be used as one of the quality control methods for Jiawei Xianglian Pills.