深部曲霉菌感染是引起临床免疫低下患者感染及病死较严重的条件致病菌之一。传统的实验室诊断存在一定的弊端。现从血清学和分子生物学两方面对曲霉菌诊断的现状及优缺点,以及Real-timePCR诊断的发展前景及存在的问题作一综述。 Aspergillus infection is caused by clinical immunocompromised patients with severe infection and death of one of the conditions of pathogens. Traditional laboratory diagnosis has some disadvantages. The current status, advantages and disadvantages of Aspergillus diagnosis, as well as the development prospects and existing problems of Real-time PCR diagnosis are summarized from the aspects of serology and molecular biology.