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目的本实验以研究麻疹病毒（ＭＶ）为目的，采用反转录聚合酶链式反应（ＲＴＰＣＲ），早期、快速、准确、灵敏地检出ＭＶ，通过对扩增产物的基因分析，判断传染源和传播途径，并研究ＭＶ基因型。方法对１９９５年６月大连地区发生的一起５人的麻疹流行进行ＭＶ检测，用反转录套式ＰＣＲ法分别扩增ＭＶ的核蛋白基因（Ｎ）和基质蛋白基因（Ｍ），阳性对照用长春冻干麻疹活疫苗。对疫苗株长－４７（９４０５）和任意选取的两个标本ＤＬ１Ｃｈｉｎａ９５、ＤＬ２Ｃｈｉｎａ９５的扩增片段进行核酸序列测定，并对核酸序列、相应的氨基酸序列及基因差异进行计算机分析。结果疫苗株和患者标本均扩增得到Ｎ基因３１８ｎｔ，Ｍ基因１８８ｎｔ核酸片段，确证此次为麻疹流行。ＤＬ１Ｃｈｉｎａ９５、ＤＬ２Ｃｈｉｎａ９５有完全相同的核酸序列，属同一流行株，说明此次麻疹流行来自同一传染源，此结果与流行病调查结果相符。此流行株不同于疫苗株长－４７（９４０５）。Ｍ基因的１５２ｎｔ中，流行株５ｎｔ不同于疫苗株，其中有义突变２ｎｔ。Ｎ基因的变异较大，在２８２ｎｔ中，流行株有２１ｎｔ不同于疫苗株，其中有义突变１２ｎｔ。依据Ｎ基因１２３５～１５１６核酸序列，此流行株的基因型分析结果与非洲、欧?
ObjectiveTo study the effect of measles virus (MV), reverse transcriptase-polymerase chain reaction (RTPCR) was used to detect MVs early, rapidly, accurately and sensitively. By analyzing gene products of amplification products, And route of transmission, and study MV genotypes. Methods A total of 5 human measles epidemics were detected in Dalian area in June 1995. The nucleoprotein (N) and matrix protein gene (M) of MV were amplified by reverse transcription nested PCR. The positive control Changchun freeze-dried measles live vaccine. The length of vaccine strain -47 (9405) and the randomly selected amplified fragments of two samples DL1China95 and DL2China95 were determined. The nucleic acid sequences, corresponding amino acid sequences and genetic differences were analyzed by computer. Results Both the vaccine strain and the patient samples were amplified with 318 nt of N gene and 188 nt of M gene, confirming the prevalence of measles. DL1China95, DL2China95 have the same nucleic acid sequence, belong to the same epidemic strain, indicating that the epidemic of this measles comes from the same source of infection, and the result is consistent with the epidemiological survey results. This popular strain is different from the vaccine strain length -47 (9405). Of the 152nt M genes, the 5nt strain was different from the vaccine strain, with a 2nt sense mutation. The variation of N gene is larger. In 282nt, 21nt of the epidemic strain is different from the vaccine strain, in which the 12nt sense mutation. According to the N gene 1235 ~ 1516 nucleic acid sequence, genotype analysis of this popular strain and Africa, Europe?