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目的 确定青藏高原地区藏族人群包虫病患者的人类白细胞抗原-DRB1(Human leukocyte antigen-DRB1,HLADRB1)等位基因中易感基因和抗性基因,为研究藏族人群包虫病的感染机制及遗传特点提供依据。方法 本研究采用病例对照法。病例组选取青海省玉树和果洛藏族自治州的世居藏族囊型包虫病63例和泡型包虫病73例;对照组选取该地区无血缘关系的藏族健康人,共计60例。应用聚合酶链反应-直接碱基序列基因分型(PCR-SBT)技术,比较等位基因频率。结果 泡型和囊型包虫病病例组HLA-DRB1*04等位基因频率均小于对照组(χ~2=4.71、4.31,P均<0.05)。结论HLA-DRB1*04等位基因与青藏高原藏族人群囊型和泡型包虫病相关联,对其感染有保护作用,是其抗性基因。
Objective To determine the susceptibility genes and resistance genes of human leukocyte antigen-DRB1 (HLA-DRB1) alleles in Tibetan patients in the Tibetan Plateau. To investigate the mechanism of infection and inheritance of hydatid disease in Tibetan population Features provide the basis. Methods This study used a case-control method. In the case group, 63 cases of native Tibetan cystic echinococcosis and 73 cases of vacuolar echinococcosis were selected from Yushu and Guoluo Tibetan Autonomous Prefecture in Qinghai Province. In the control group, 60 healthy Tibetan healthy people without relatives were selected. Polymerase chain reaction-direct nucleotide sequencing (PCR-SBT) was used to compare allele frequency. Results The frequencies of HLA-DRB1 * 04 alleles in the cases of cystic echinococcosis and cystic echinococcosis were all less than those in the control group (χ ~ 2 = 4.71, 4.31, P <0.05). Conclusion The HLA-DRB1 * 04 allele is associated with cystic and alveolar hydatid disease in Tibetans of the Tibetan Plateau and has a protective effect on the infection. It is a resistance gene.