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目的探讨慢性鼻窦炎筛窦勃膜组织中GAT:A-3与局部细胞因子(IL-5 , IL-6 , IL-8 )表达的关系。方法采用酶联免疫吸附测定法(enzyme-linked immunusorbent assay,ELISA)检测45例慢性鼻窦炎筛窦戮膜〔根据是否伴变应性鼻炎(allergic rhinitis , AR ),分成伴有AR(AR组,28例)和不伴有AR(NAR组,17例)两组」与11例因脑脊液鼻漏、视神经管减压而行鼻内镜手术患者的筛窦私膜(对照组)组织中IL-5 , IL-6和IL-8的含量。半定量反转录一聚合酶链反应(reverse transcription-polymerase chain reaction, RT-PCR)和免疫组织化学法检测GATA-3在筛窦勃膜中的表达,分析GATA-3表达与局部细胞因子含量间的相关性。结果3组患者筛窦赫膜组织中AR组与NAR组的IL-S,IL}b和IL-8水平均高于对照组,差异具有统计学意义(P值均<0. OS ) ; AR组的3种细胞因子水平均高于NAR组,差异具有统计学意义(P值均<0. 01)。半定量RT-PCR所得的相对密度显示AR组和NAR组的GATA-3 mRNA水平高于对照组(P值均<0. 01),同时AR组的GATA-3 mRNA水平又高于NAR组,差异具有统计学意义(Y<0.01)。免疫组化结果显示,GA’Z’A一主要在细胞质内表达,且G.4TA一阳性细胞主要为砧膜下的炎症浸润细胞。平均胞质染色阳性率AR组为(27. 92士16. 75 ) % , NAR组为(10. 22士0.05)%,对照组为(1. 30士1. 78 ) % o AR组和NAR组的慢性鼻窦炎患者GATA-3的胞质阳性率高于对照组,差异具有统计学意义(P值均0.05)结论慢性鼻窦炎中GATA-3激活是诱导局部IL-5表达的可能机制之一,变应性鼻炎的存在可以进一步提高GATA一的活性,进而增加局部IL-5的表达。慢性鼻窦炎的发生发展与变态反应有一定的关系,GATA-3在慢性鼻窦炎的发病机理中可能是关键因素之一。ILK和IL-8的局部表达不依赖于GATA-3途径。
Objective To investigate the relationship between GAT: A-3 and the expression of local cytokines (IL-5, IL-6, IL-8) in the sinous sinus of chronic sinusitis. Methods The ethmoidal mucosal membrane of 45 patients with chronic sinusitis was detected by enzyme-linked immunosorbent assay (ELISA) according to whether it was accompanied by allergic rhinitis (AR) 28 cases) and no AR (NAR group, 17 cases) and 11 cases of nasal endoscopic sinus surgery due to cerebrospinal fluid rhinorrhea and optic canal decompression in patients with ethmoid sinus membrane (control group) IL- 5, IL-6 and IL-8 levels. The expression of GATA-3 in ethmoid sinus was detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. The expression of GATA-3 and the expression of local cytokines Relevance between. Results The levels of IL-S, IL-b and IL-8 in AR and NAR group were significantly higher than those in control group (all P values <0. OS) The levels of the three cytokines in the group were higher than those in the NAR group, with statistical significance (P <0.01). The relative density of semi-quantitative RT-PCR showed that GATA-3 mRNA level in AR group and NAR group was higher than that in control group (P <0.01), meanwhile GATA-3 mRNA level in AR group was higher than that in NAR group The difference was statistically significant (Y <0.01). The results of immunohistochemistry showed that GA’Z’A was mainly expressed in the cytoplasm, and G.4TA-positive cells were mainly inflammatory infiltrating cells under the antrum. The average positive rate of cytoplasmic staining was (27. 92 ± 16.75)% in AR group, (10.22 ± 0.05)% in NAR group, and (1.30 ± 1.78)% in AR group and NAR The positive rate of GATA-3 in the group of chronic sinusitis was higher than that of the control group (P <0.01), and the AR group was higher than that of the NAR group, the difference was statistically significant (P < 0. 01) o Pearson correlation analysis showed that there was a correlation between the positive rate of cytoplasmic staining of GATA-3 and the local IL-5 level in patients with sinusitis (r = 0.712, P <0.01) a GATA-3 cells There was a lack of correlation between the expression of IL-6 and IL-8 (r = 0.200 and 0. 089, respectively, P> 0.05) .Conclusion GATA-3 activation in chronic sinusitis induces local IL-5 expression One of the possible mechanisms, the presence of allergic rhinitis can further increase the activity of GATA, thereby increasing the expression of local IL-5. The occurrence and development of chronic sinusitis and allergy have a certain relationship, GATA-3 in the pathogenesis of chronic sinusitis may be one of the key factors. Local expression of ILK and IL-8 is independent of the GATA-3 pathway.