血清核糖核酸酶(RNase)应用于临床已日益引起人们重视。因此选择一个简便易行测定方法已成为进一步发挥其效益的先行任务。依我室条件确立了下列测试方法,在1小时内即可完成。此法的原理是:血清RNase能水解大分子多核苷酸而生成寡核苷酸和单核苷酸。离心沉淀弃去大分子核糖核酸,在260nM测上清液中寡核苷酸和单核苷酸量,即可知酶的活力。 Serum RNase (RNase) has been used clinically for more and more attention. Therefore, the choice of a simple and easy to determine the method has become to further play its effectiveness of the first task. According to my room conditions established the following test methods, in 1 hour to complete. The principle of this method is that serum RNase can hydrolyze macromolecular polynucleotides to generate oligonucleotides and single nucleotides. Centrifugal sedimentation to remove macromolecular ribonucleic acid, 260nM in the supernatant of the oligonucleotide and mononucleotide amount, we can see that the enzyme activity.