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优化肝水解肽体外生物学活性检测方法。调整L02细胞浓度到8×104个/ml,以100 l每孔接种于96孔板中,37℃、5%CO2条件下贴壁时间为24 h,弃去旧的培养基,加入以不含谷氨酰胺和胎牛血清的RPMI 1640培养基稀释的肝水解肽溶液,作用48 h。优化后的方法相对于原方法显著提高了肝水解肽促进L02细胞生长的量效反应关系,能定量评价肝水解肽体外活性。
Optimization of in vitro bioactivity assay of hepatic hydrolytic peptide. Adjust the concentration of L02 cells to 8 × 104 cells / ml and inoculate them in 96-well plates at 100 μl per well. The attachment time is 24 h at 37 ° C under 5% CO2. Discard the old medium and add Glutamine and fetal bovine serum in RPMI 1640 medium diluted hepatic hydrolyzate solution for 48 h. Compared with the original method, the optimized method can significantly improve the dose-response relationship of the liver hydrolyzing peptide to promote the growth of L02 cells, and can quantitatively evaluate the in vitro activity of the liver hydrolyzing peptide.