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为了探讨酸雨胁迫与毛竹(Phyllostachys pubescens)绿叶挥发物(green leaf volatiles,GLVs)释放规律以及抗氧化酶活性的关系,通过盆栽试验,采用不同pH值(5.6、4.0、2.5)的模拟酸雨对毛竹三年生实生苗进行处理,研究酸雨对毛竹叶片可溶性蛋白质含量、丙二醛(MDA)含量和抗氧化酶活性的影响,并采用热脱附/气相色谱/质谱联用技术对毛竹释放的GLVs成分和含量进行分析。结果表明:酸雨胁迫下毛竹叶片MDA含量明显增加,pH 2.5模拟酸雨胁迫处理45天毛竹叶片MDA含量与对照相比增加了43.0%(p<0.01);pH 4.0处理MDA含量增加缓慢,处理75天时MDA含量比对照增加了0.36倍(p<0.01)。pH 4.0和pH 2.5模拟酸雨胁迫处理45天时,毛竹叶片可溶性蛋白质含量极显著增加,与对照相比分别增加了32.0%和65.0%(p<0.01)。在酸雨胁迫下,毛竹叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的响应时间存在一定差异,表现为互相协调,pH 2.5模拟酸雨胁迫处理SOD活性和POD活性分别在45天和60天时达到最大值,分别为对照的1.67倍和1.31倍(p<0.01),随后降低。pH 4.0和pH 2.5模拟酸雨胁迫处理,毛竹叶片GLVs含量比对照分别增加26.4%和132.9%(p<0.01),新增GLVs为(E)-2-辛烯醛、2-乙基己醛、(E)-2-己烯醛和(E)-2-壬烯醛。研究表明:酸雨胁迫条件下,毛竹可以通过调节保护酶活性、可溶性蛋白质含量和释放GLVs来提高适应环境的能力。
In order to investigate the relationship between acid rain stress and the release of green leaf volatiles (GLVs) and the activities of antioxidant enzymes in Phyllostachys pubescens, pot experiments were conducted to study the effects of acid rain with different pH values (5.6, 4.0 and 2.5) The three-year-old seedlings were treated to study the effects of acid rain on soluble protein content, malondialdehyde (MDA) content and antioxidant enzyme activities in leaves of Phyllostachys pubescens. The contents of GLVs released by Phyllostachys pubescens were determined by thermal desorption / gas chromatography / mass spectrometry And content analysis. The results showed that the content of MDA in leaves of Phyllostachys edulis increased significantly under the acid rain stress. The content of MDA in Phyllostachys pubescens leaves increased by 43.0% (P <0.01) at pH 2.5 for 45 days. The content of MDA increased slowly at pH 4.0. MDA content increased by 0.36 times than the control (p <0.01). At pH 4.0 and pH 2.5 for 45 days, the content of soluble protein in leaves of Phyllostachys edulis increased significantly, increased by 32.0% and 65.0% (p <0.01) compared with the control. Under the acid rain stress, the response time of SOD, CAT and POD of Phyllostachys pubescens leaves had some differences, which showed that they were coordinated with each other. PH 2.5 simulated acid rain stress treatment SOD Activity and POD activity reached their maximum at 45 and 60 days, respectively, 1.67 and 1.31 fold (p <0.01), respectively, of the control, followed by a decrease. Compared with the control, the content of GLVs in leaves of Phyllostachys pubescens increased by 26.4% and 132.9%, respectively (p <0.01) at pH 4.0 and pH 2.5. The newly added GLVs were (E) -2-octenal, (E) -2-hexenal and (E) -2-nonenal. The results showed that under the condition of acid rain stress, Moso bamboo can improve the ability to adapt to the environment by regulating the activities of protective enzymes, soluble protein and release of GLVs.