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目的 为进一步研究静脉吸毒者 (IVDUs)中丙型肝炎病毒 (HCV)感染的特点。方法 采用ELISA法对114份IVDUs,其中包括人类免疫缺陷病毒 (HIV)阳性50份 ,HIV阴性64份,以及118份非静脉吸毒者 (NIVDUs)血清进行抗HCV检测 ;并采用RT-PCR法对其中抗HCV阳性者和40例抗HCV阳性的非吸毒者血清用HCVⅡ型引物进行HCV-RNA检测。 结果 HIV阳性的IVDUs抗HCV阳性率94.0% (47/50) ,HCV -RNA阳性率56.0% (28/50) ;HIV阴性的IVDUs抗HCV阳性率21.9% (14/64) ,HCV-RNA阳性率为28.6% (4/14) ;抗HCV阳性非吸毒者HCV -RNA阳性率为45.0% (18/40)。结论 静脉吸毒是HCV感染的高危因素 ;HIV阳性的IVDUs中HCV感染率极高 ;IVDUs的HCV重叠HIV感染可使HCV-RNA阳性率增高 ,提示在IVDUs中HIV可能促进了HCV的复制
The purpose is to further investigate the characteristics of hepatitis C virus (HCV) infection in intravenous drug users (IVDUs). Methods ELISA was used to detect anti-HCV in 114 IVDUs, including 50 HIV-positive, HIV-negative 64, and 118 serum from non-intravenous drug users (NIVDUs). RT- Among them, anti-HCV positive and 40 anti-HCV positive non-addicted sera were detected by HCV type II HCV-RNA. Results The positive rate of HCV-RNA was 94.0% (47/50) in HIV-positive IVDUs and 56.0% (28/50) in HIV-positive IVDUs. The positive rate of HIV-negative IVDUs was 21.9% (14/64) The rate was 28.6% (4/14). The positive rate of HCV-RNA in anti-HCV positive non-drug users was 45.0% (18/40). Conclusion Intravenous drug use is a high risk factor for HCV infection. HCV infection rates among HIV-positive IVDUs are extremely high. HCV co-infection with IVDUs can increase HCV-RNA positive rate, suggesting that HIV may promote HCV replication in IVDUs