福尔马林固定时,组织中的许多氨基酸残基在分子内或分子间形成了醛键,使得不少抗原决定簇被封闭。用胰蛋白酶、尿素等,通过机械的方法断开因福尔马林固定的导致的抗原表位和不相关蛋白间的交联键,使被掩盖的抗原决定簇或变性的抗原重新暴露或抗原性得到一定程度的恢复,使抗体更好的渗透,与表位结合。因此,抗原修复是影响免疫组化染色结果的基本因素。本实验室多年来一直采用肾穿组织石蜡切片Eli Vision When formalin is immobilized, many of the amino acid residues in the tissue form aldehyde bonds either intramolecularly or intermolecularly, allowing many of the epitopes to be blocked. The masked epitopes or denatured antigens are re-exposed or antigenized by tryptase, urea or the like by mechanical means to break the crosslinks between antigenic epitopes and irrelevant proteins due to formalin fixation Sex has been restored to some extent, allowing better penetration of antibodies and binding to epitopes. Therefore, antigen repair is the basic factor affecting the immunohistochemical staining results. For many years, our laboratory has used Eli Vision, a paraffin-embedded, renal tissue paraffin section