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目的:观察化纤汤对C57BL小鼠急性放射性肺损伤(acute radiation-induced lung injury,ARILI)的治疗效果,并探讨其机制。方法:将20只C57BL小鼠随机分为0.9%氯化钠溶液组和放射+化纤汤组,每组10只小鼠。采用60Coγ射线单次16Gy大剂量照射小鼠全肺建立ARILI模型。照射后第二天化纤汤组用化纤汤灌胃,0.9%氯化钠溶液组用等体积0.9%氯化钠溶液灌胃,连续灌胃30天。取小鼠肺组织进行组织病理学观察和胶原纤维染色。采用ELISA法检测小鼠血清转化生长因子β1(transforming growth factor-β1,TGF-β1)和白细胞介素6(interleukin-6,IL-6)水平。结果:化纤汤组小鼠肺组织损伤程度较对照组小鼠明显减轻,胶原沉积减少,其血清TGF-β1和IL6表达水平较对照组相比明显降低,差异有统计学意义(P<0.05)。结论:化纤汤可有效减轻小鼠的ARILI,且对小鼠肺组织几乎无毒性,具有广阔的临床应用前景。
Objective: To observe the therapeutic effect of Huaxi Decoction on C57BL mice with acute radiation-induced lung injury (ARILI) and to explore its mechanism. Methods: 20 C57BL mice were randomly divided into 0.9% sodium chloride solution group and radiation + chemical fiber soup group, each group of 10 mice. The ARILI model was established by whole lung irradiation with a single dose of 16 Gy in 60Coγ-rays. The next day after irradiation, the chemical fiber soup group was fed with chemical fiber soup. The 0.9% sodium chloride solution group was orally administered with an equal volume of 0.9% sodium chloride solution for 30 days. Mouse lung tissue was taken for histopathological observation and collagen staining. Serum levels of transforming growth factor-β1 (TGF-β1) and interleukin-6 (IL-6) were measured by ELISA. Results: Compared with the control group, the damage of lung tissue in the Huaxian Decoction group was significantly reduced and the collagen deposition was reduced. The expression of TGF-β1 and IL-6 in the model group was significantly lower than that in the control group (P <0.05) . Conclusion: Chemical Fiber Decoction can effectively reduce the ARILI in mice, and it is almost non-toxic to mouse lung tissue. It has broad clinical application prospect.