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目的 确定汉滩病毒（ＨＴＮＶ）上可与宿主细胞受体结合的一段配基表位。方法 型特异性ｍＡｂ ３Ｇ１，与 ＨＴＮＶ糖蛋白ＧＺ及核蛋白（ＮＰ）具有双结合活性，且中和效价很高。以纯化的 ｍＡｂ ３Ｇ１作为配基，淘筛噬菌体随机 １２肽库，经ＥＬＩＳＡ法鉴定后，对阳性克隆进行测序并与天然病毒蛋白Ｇ２及ＮＰ的序列进行同源性比较分析。结果 经３轮筛选，噬菌体显示有良好的富集效果。从第３轮筛选产物中，随机挑取２１个克隆进行ＥＬＩＳＡ结合实验。结果显示，９个克隆与 ｍＡｂ３Ｇ１有较强的特异结合活性；ＤＮＡ测序结果表明，具有保守性序列模式ＰＸ＿（１－２）ＨＸ＿（０－２）。该基序分别与Ｇ２～（９６）ＹＰＷＨＴＫＣＨＹ～（１０５）及 ＮＰ～（８１）ＰＹＧＶＥＰＧＤＨＬＫＥＲＳ～（９４）相似。结论 从噬菌体随机肽库中，筛选到能与ｍＡｂ ３Ｇ１特异性结合的一段短肽基序 ＰＸ＿（１－２）ＨＸ（０－２），其与 ＨＴＮＶ Ｇ２部分氨基酸的同源性较高，可能是与宿主细胞受体结合的表位，为进一步证实和研究ＨＴＮＶ的受体奠定了基础。
Objective To determine a ligand epitope on the Hantaan virus (HTNV) that binds to host cell receptors. Method-specific mAb 3G1 has dual binding activity with HTNV glycoprotein GZ and nucleoprotein (NP), and the neutralization titer is high. The purified mAb 3G1 was used as a ligand to screened the random peptide library of phage. After identification by ELISA, the positive clones were sequenced and compared with the sequences of native virus proteins G2 and NP. Results After 3 rounds of screening, phage showed a good enrichment effect. From the third round of screening, 21 clones were randomly selected for ELISA binding experiments. The results showed that 9 of the clones had strong specific binding activity with mAb3G1. The results of DNA sequencing showed that there was a conserved sequence pattern of PX_ (1-2) HX_ (0-2). The motif was similar to G2 ~ (96) YPWHTKCHY ~ (105) and NP ~ (81) PYGVEPGDHLKERS ~ (94), respectively. Conclusion A short peptide motif PX_ (1-2) HX (0-2) that specifically binds to mAb 3G1 was screened from the phage random peptide library and has high homology with some amino acids of HTNV G2, possibly Is an epitope that binds to the host cell receptor, which lays the foundation for further confirmation and study of HTNV receptor.