巨细胞病毒(CMV)已逐渐被认为是人类重要致病因子之一。除能引起各种临床综合征外,还可在体外致田鼠和人细胞转化,提示具有致癌的潜在能力。本文报告,自16例结肠腺癌细胞培养中分出3株巨细胞病毒。作者将16例分布于不同部位的结肠腺癌外科标本洗涤处理剪碎后,置含10%胎牛血清基础培养基的组织培养瓶中37℃培养。待长成完整单层细胞后即传代。细胞系按常规法置入液氮(-196℃)保存。若该细胞培养出现细胞病变(CPE),则收取细胞和上清液接种到人胚肺纤维母细胞(HEF),并用下列方法鉴定病毒:(1)感染细胞做超 Cytomegalovirus (CMV) has gradually been considered as one of the important pathogens in human. In addition to causing a variety of clinical syndromes, but also in vitro voles and human cells transformed, suggesting the potential of cancer-causing ability. This report reports three cytomegalovirus strains isolated from 16 colon adenocarcinoma cell lines. The authors screened 16 surgical specimens of colon adenocarcinoma distributed in different sites and then cultured in 37 ° C tissue culture flasks containing 10% fetal bovine serum basal medium. To be grown into a complete monolayer of cells after passage. Cell lines by conventional methods into liquid nitrogen (-196 ℃) preservation. If cytopathic effect (CPE) occurs in this cell culture, the cells and supernatant are collected and inoculated into human embryonic lung fibroblasts (HEFs), and the virus is identified by the following methods: (1)