培养基制作: 营养肉汤粉1.3克葡萄糖 1克 L-盐酸胱氨酸0.2克蒸馏水90毫升灭活混合人血清10毫升,1％氯霉素溶液1毫升将营养肉汤粉、葡萄糖和盐酸胱氨酸溶于蒸馏水中,121℃灭菌15分钟。再将灭活人血清和氯霉素用无菌手续加入上述溶液内。充分混合后,以1mol/NaOH调PH至6.4。将此培养基分装于已灭菌的带螺丝盖的瓶内,每瓶2～3毫升。在4℃下可存放6周。 Medium Preparation: Nutrient broth powder 1.3 g Glucose 1 g L-Cystine 0.2 g Distilled water 90 ml Inactivated mixed human serum 10 ml, 1% chloramphenicol solution 1 ml Nutrient broth powder, glucose, and hydrochloric acid Amino acids were dissolved in distilled water and sterilized at 121 ° C for 15 minutes. Inactivated human serum and chloramphenicol were then aseptically added to the solution. After thorough mixing, adjust pH to 6.4 with 1 mol / NaOH. This medium was dispensed in sterilized bottles with screw caps, 2 to 3 ml per bottle. Store at 4 ° C for 6 weeks.