基于HPLC-MS/MS法的补肾益智方君药蛇床子素抗阿尔茨海默病药动学

来源 :中国药理学与毒理学杂志 | 被引量 : 0次 | 上传用户：mjynht

【摘要】

：

目的考察补肾益智方君药蛇床子素的药动学,探究复方增强蛇床子素生物利用度的机制。方法通过构建HPLCMS/MS高灵敏度的蛇床子素准确定量分析方法,以多反应检测(MRM)方式,以欧前胡素为内标,建立蛇床子和欧前胡素的母离子(Q1)和子离子(Q3)的离子对;进一步优化HPLC-MS/MS参数,实现蛇床子素的准确定量分析。以SD大鼠为模型动物,通过ig给药方式,设置对照组、假手术组和手术组,用连续给药的方式,眼眶采血,时间点为0.5,1,2,4,6,8,10,12和24 h,基于HPLC-MS/MS测定蛇床子素的

【作者】

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梁勇刘金满伍帆昌黄明俊王奇

【机构】

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广州中医药大学科技创新中心,广州中医药大学临床药理研究所

【出处】

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中国药理学与毒理学杂志

【发表日期】

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2021年10期

【关键词】

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阿尔茨海默病补肾益智方蛇床子素 HPLC-MS/MS 药动学

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