论文部分内容阅读
近年的研究发现,肿瘤细胞存在Th2型细胞因子偏移的倾向.我们选择了20种人肿瘤细胞株,采用RT-PCR和RNA斑点杂交的方法分析Th1/Th2类细胞因子的分布情况.20种人肿瘤细胞株中包括11株实体瘤:以IL-2、IFN-γ代表Th1类细胞因子,IL-4、IL-10、IL-13代表Th2类细胞因子,β-actin作为内参照,并初步进行了半定量估计.11株实体瘤中,除HTB-133T-47D和小细胞肺癌有Th1类细胞因子表达,呈现混合的ThO型,M14无细胞因子表达外,其余8株均呈典型的Th2型细胞因子分泌状态,并且二株ThO型在表达量上亦明显的向Th2类偏移,为ThOTh2型.9株血液系统肿瘤表现的结果较复杂,除Karpas淋巴瘤和U266骨髓瘤表达典型的Th2型细胞因子外,其余均为混合表达的ThO型
Recent studies have found that there is a tendency for Th2 cytokines to shift in tumor cells. We selected 20 human tumor cell lines and analyzed the distribution of Th1/Th2 cytokines using RT-PCR and RNA dot blot methods. There are 11 solid tumors in human tumor cell lines: IL-2, IFN-γ represent Th1 cytokines, IL-4, IL-10, IL-13 represent Th2 cytokines, β-actin serves as an internal reference, and A semi-quantitative estimate was performed. Among the 11 solid tumors, except for HTB-133T-47D and small cell lung cancer, Th1 cytokines were expressed and mixed ThO types were present. M14 showed no cytokines, and the remaining 8 strains were typical. Th2 cytokine secretion status, and the two strains of ThO type also obviously shifted to Th2 type in the expression quantity, and the result of ThOTh2 type 9 strain hematological malignancy was more complicated, except for the expression of Karpas lymphoma and U266 myeloma. The Th2-type cytokines, the rest are mixed expression of ThO type