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[目的]分析TRB3基因+251A/G多态性分布,并探讨该多态性与糖耐量减低(IGT)及胰岛素抵抗的相关性。[方法]在112例IGT者和209例对照者中,采用Taqman探针方法对TRB3基因+251A/G位点进行基因分型;同时进行问卷调查、体格检查及血糖、血脂、胰岛素等生化指标的检测,并计算稳态模型法β细胞功能指数(HOMA-B)、早期相胰岛素分泌指数(ΔI30/ΔG30)、稳态模型法胰岛素抵抗指数(HOMA-IR)、Cederholm胰岛素敏感指数(ISIc)等评价胰岛素分泌及胰岛素敏感性。[结果]在IGT组与对照组中,性别、年龄差异无统计学意义;两组临床指标比较:BMI、血糖(空腹及糖后2 h)、糖化血红蛋白、游离脂肪酸、胰岛素(空腹及糖后2 h)、ΔI30/ΔG30、HOMA-IR、ISIc等10项差异有统计学意义。TRB3基因+251A/G位点存在多态性。AA、AG、GG 3种基因型的频率分布在IGT组、对照组分别为64.29%、64.11%,28.57%、32.54%,7.14%、3.35%,其分布差异无统计学意义(χ~2=0.006,P=0.938)。两组不同基因型的临床指标比较:在IGT组中,AG/GG基因型者空腹胰岛素、HOMA-IR高于AA基因型者(P<0.05);对照组+251A/G不同基因型者血糖、血脂、胰岛素、HOMA-B、HOMA-IR等各指标差异均无统计学意义。[结论]TRB3基因+251A/G多态性与研究人群IGT的发生无关,但可能与IGT者肝脏胰岛素抵抗存在相关性。
[Objective] To analyze the distribution of + 251A / G polymorphism of TRB3 gene and explore the relationship between the polymorphism and impaired glucose tolerance (IGT) and insulin resistance. [Methods] Taqman probe was used to genotype the + 251A / G locus of TRB3 gene in 112 IGT patients and 209 healthy controls. At the same time, questionnaires, physical examination and biochemical indexes such as blood glucose, blood lipid, insulin and so on (HOMA-B), early phase insulin secretion index (ΔI30 / ΔG30), homeostasis model insulin resistance index (HOMA-IR), Cederholm insulin sensitivity index (ISIc) Evaluation of insulin secretion and insulin sensitivity. [Results] There were no significant differences in sex and age between the IGT group and the control group. The clinical indexes of the two groups were compared: BMI, blood glucose (fasting and 2 h), glycosylated hemoglobin, free fatty acids, insulin 2 h), ΔI30 / ΔG30, HOMA-IR, ISIc and other 10 differences were statistically significant. TRB3 gene + 251A / G polymorphism. The frequencies of AA, AG and GG genotypes were 64.29%, 64.11%, 28.57%, 32.54%, 7.14% and 3.35% in the IGT group and the control group respectively, with no significant difference in distribution (χ ~ 2 = 0.006, P = 0.938). In IGT group, fasting insulin and HOMA-IR were higher in AG / GG genotype than those in AA genotype (P <0.05); in control group, 251A / G genotype , Blood fat, insulin, HOMA-B, HOMA-IR and other indicators showed no significant difference. [Conclusion] The + 251A / G polymorphism of TRB3 gene is not related to the occurrence of IGT in the study population, but may be related to the hepatic insulin resistance in IGT.