论文部分内容阅读
本研究利用59对SSR引物对吉853、黄早四、自330等8份玉米自交系进行了分析,结果表明:吉853和黄早四之间的遗传相似性最大(GS=0.71),与自330有较大的遗传差异(GS=0.55)。黄早四和自330在吉853中的等位基因流向分为4种类型,第1种是3个自交系带型均相同,占26.67%,属于未发生重组位点;第2种是来自黄早四的等位基因,占33.33%;第3种是来自自330的等位基因,只占11.67%;第4种是与黄早四和自330均不同的带型,属变异重组的位点,占28.33%,且有集中分布的趋势,主要集中于4个区段(Bin2.0-Bin2.03、Bin3.02-Bin3.05、Bin5.01-Bin5.04和Bin8.08),这些重组变异也大大丰富了吉853的遗传多样性。本研究证明了在二环选系重组过程中也会发生相当程度的变异,表明二环选系在今后依然是玉米育种的重要方法。
In this study, 59 pairs of SSR primers were used to analyze 8 maize inbred lines, Ji 853, Huangzao 4 and Chu 330. The results showed that the genetic similarity between Ji 853 and Huangzao 4 was the highest (GS = 0.71) There was a large genetic difference from 330 (GS = 0.55). Huangzao four and from 330 in Ji 853 allele flow is divided into four types, the first is the three inbred lines are the same, accounting for 26.67%, belonging to no recombination sites; the second is 33.33% of the alleles from Huangzao IV, 3 of the alleles from 330, accounting for only 11.67%, and the 4 kinds of alleles were different from those of Huangzao IV and 330 since they belong to mutation recombination , Accounting for 28.33% of the total, with a trend of concentration distribution, mainly concentrated in 4 sectors (Bin2.0-Bin2.03, Bin3.02-Bin3.05, Bin5.01-Bin5.04 and Bin8.08 ), These recombinant variation also greatly enriched the genetic diversity of Kat 853. This study demonstrates that considerable variation can occur during the reorganization of the second ring system, indicating that the second ring system remains an important method of maize breeding in the future.