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雄性小鼠分别经饮水染毒0、1000、3162和10000ppm的醋酸铅,染毒后第2周、第7周和第12周每组随机抽取5只雄鼠眼眶取血并分离附睾,进行血铅及精子计数、活动度、形态检查。染毒期内对照组小鼠血铅无明显变化,实验组血铅于染毒第2周即明显增高,并随染毒时间进一步上升,呈良好的剂量效应关系;染毒第2周末,1000和3162ppm组即见精子活动能力和存活率明显下降(P<0.05),其他指标无明显改变;染毒第7周末见3162和10000ppm组精子头部畸形率明显增高(P<0.05);染毒第12周末1000ppm组畸形精子率也见增高,而精子计数下降仅见于10000ppm组长期染毒后。提示醋酸铅可影响小鼠精子发生,其中小鼠精子活力改变是铅生殖毒性最敏感的指标,其次为精子形态改变,而精子计数仅于长期接触高浓度铅方出现改变。
Male mice were exposed to drinking water at 0, 1000, 3162, and 10000 ppm of lead acetate, respectively. At the second, seventh, and twelfth week after the exposure, five male mice were randomly selected to take blood from the eyelids and the epididymis was isolated to conduct blood lead. Sperm count, activity, morphology check. There was no significant change in blood lead in mice of the control group during the exposure period. Blood lead in the experimental group increased significantly during the first 2 weeks of exposure, and further increased with the exposure time, showing a good dose-effect relationship; at the second weekend of exposure, 1000 In the group of 3162ppm, sperm activity and survival rate were significantly decreased (P<0.05). No other changes were found in other indicators. In the 7th week after exposure, the abnormal rate of sperm in the 3162 and 10000ppm groups was significantly higher (P<0.05); At the end of the 12th week, the rate of abnormal spermatozoa in the 1000ppm group also increased, but the decrease in sperm count was only observed after the long-term exposure to the 10000ppm group. It is suggested that lead acetate can affect spermatogenesis in mice, in which changes in mouse sperm motility are the most sensitive indicators of lead reproductive toxicity, followed by sperm morphological changes, and sperm counts change only after prolonged exposure to high concentrations of lead.