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目的探讨低Se、低VE是否在心肌损伤后的修复过程中持续发挥作用,观察适时适量补充Se和VE,是否可减轻和逆转过度的心肌胶原蓄积。方法 Wistar大鼠30只,随机取6只大鼠做正常对照组,采用混合饲料喂养。其他大鼠均采用低Se、低VE半合成饲料喂养。81 d后,除正常对照组外,其余大鼠注射异丙基肾上腺素制备偏食大鼠心肌纤维化模型。制模成功大鼠分为4组,半合成饲料组(EG)继续采用半合成饲料喂养。其余各组膳食中施加干预如下:半合成饲料加Se组、半合成饲料加VE组、半合成饲料加硒加VE组。喂养21d后,乙醚麻醉,眼球采血,取大鼠心脏,观察大鼠氧化应激指标和心肌胶原代谢情况。结果偏食心肌纤维化模型组大鼠血清中谷胱甘肽过氧化物酶活力下降,脂质过氧化物含量升高。心肌胶原蓄积,并伴有金属基质蛋白酶-2、金属基质蛋白酶抑制剂-2表达上调、两者比值降低。膳食中补充Se、联合补充Se和VE均可一定程度的缓解这些改变。结论低Se、低VE在心肌损伤后的修复过程中持续发挥作用,适时适量的补充Se和VE有利于减轻和逆转过度的心肌胶原蓄积。
Objective To investigate whether low Se and low VE continue to play a role in the repair process of myocardial injury and to observe whether adequate and timely replenishment of Se and VE can reduce and reverse the excessive accumulation of myocardial collagen. Methods Thirty Wistar rats were randomly divided into six groups: normal control group and fed with mixed diet. The other rats were fed low Se, low VE semi-synthetic feed. After 81 days, except the normal control group, the other rats were injected isoprenaline to prepare the rat model of myocardial fibrosis. The successful model rats were divided into 4 groups, semi-synthetic feed group (EG) continue to feed semi-synthetic feed. The rest of the dietary interventions were as follows: semi-synthetic feed plus Se group, semi-synthetic feed plus VE group, semi-synthetic feed plus selenium plus VE group. After feeding for 21 days, the rats were anesthetized with ether and the blood was taken from the eyeballs. The heart of the rats was taken out to observe the oxidative stress index and the myocardial collagen metabolism in rats. Results Serum glutathione peroxidase activity and lipid peroxidation in the model group were decreased. Myocardial collagen accumulation, and accompanied by metal matrix proteinase-2, metal matrix proteinase inhibitor-2 expression increased, the ratio between the two reduced. Dietary supplementation of Se, combined with Se and VE can alleviate these changes to some extent. Conclusions Low Se and low VE play a continuous role in the repair process of myocardial injury. The timely and appropriate supplementation of Se and VE can reduce or reverse the excessive accumulation of myocardial collagen.