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优化了磺胺甲基嘧啶(SM1)、磺胺二甲嘧啶(SM2)、磺胺对甲氧嘧啶(SMT)、磺胺甲噁唑(SMZ)4种磺胺类抗生素的高效液相色谱(H PLC)检测方法,分析了广州市养殖场周边土壤中磺胺类抗生素的残留特征,并进行了2种磺胺类抗生素的土壤降解试验。结果表明,4种磺胺类抗生素分别在0.10~10μg ml-1范围内线性良好,相关系数R>0.99。确定了最佳提取液为甲醇:含EDTA的Mcllvain缓冲液=1∶1(V/V),4种磺胺类药物的检测限与回收率分别为2.9~4.7μg kg-1、83.6%～90.1%。广州市18个规模化养殖场周边土壤中磺胺类抗生素污染以SM2为主,含量为1.75μgkg-1,其它3种均未被检出。土壤中磺胺类抗生素的含量总体呈随培养时间而不断下降的趋势,SMZ的降解速率大于SM2。
High performance liquid chromatography (H PLC) detection of four sulfonamide antibiotics such as sulfamethazine (SM1), sulfamethazine (SM2), sulfonamido-p-methoxypyrimidine (SMT) and sulfamethoxazole (SMZ) , The residual characteristics of sulfa antibiotics in the soil around the farms in Guangzhou were analyzed and the soil degradation tests of two sulfa antibiotics were carried out. The results showed that the four sulfonamide antibiotics in the range of 0.10 ~ 10μg ml-1 were good linearity, the correlation coefficient R> 0.99. The optimal extraction conditions were methanol: EDTA-containing Mcllvain buffer = 1: 1 (V / V), and the detection limits and recoveries of the four sulfonamides were 2.9-4.7 μg kg-1,83.6% -90.1 %. The contamination of sulfonamides in the soil around 18 large-scale farms in Guangzhou was dominated by SM2 with a content of 1.75 μg kg-1. The other three species were not detected. The content of sulfa antibiotics in soil generally showed a decreasing trend with the incubation time, and the degradation rate of SMZ was greater than that of SM2.