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目的通过观察内毒素血症幼年大鼠心肌降钙素基因相关肽(calcitonin gene-related peptide,CGRP)的动态变化及谷氨酰胺(glutamine,Gln)、地塞米松(dexamethosone,Dex)对其影响,探讨CGRP对内毒素血症大鼠心肌的保护作用。方法选用健康18日龄Wistar大鼠128只,按腹腔注射药物不同分为:0h对照组(Ctrl:生理盐水,n=8),内毒素血症组(LPS:n=40)、谷氨酰胺组(Gln,n=40),地塞米松组(Dex,n=40),后3组又分为2、4、6、24及72h5个时点,每个时点8只,在各指定时点分别将大鼠断头分离心脏,用放射免疫方法测定心肌CGRP表达水平。结果1.与对照组比较,各组心肌组织内CGRP均下降,P<0.05～0.01;LPS组CGRP下降最明显,最低点在6h,Gln和Dex组最低点均后延至24h,Gln降低最轻,但3组最低点之间无显著差别,P>0.05。2.在内毒素血症早期,Dex组下降最慢,但在恢复期,Gln组恢复最快,虽明显高于LPS组,但仍显著低于0h对照组,P<0.05。结论Gln和Dex具有抑制CGRP下降的作用,其中Dex抑制早期CGRP下降作用较大,而Gln促进恢复作用更明显。
Objective To observe the dynamic changes of calcitonin gene-related peptide (CGRP) and the effects of glutamine (Gln) and dexamethosone (Dex) on the myocardium in young rats with endotoxemia To investigate the protective effects of CGRP on myocardium of endotoxemic rats. Methods 128 healthy 18-day-old Wistar rats were divided into four groups according to their intraperitoneal injection: 0h control group (Ctrl: saline, n = 8), endotoxemia group (LPS: n = 40), glutamine Group (Gln, n = 40) and dexamethasone group (Dex, n = 40). The latter three groups were further divided into 2 groups of 4, 6, 24 and 72 h at 5 time points, At the time point, the heart was decapitated and the expression of CGRP in myocardium was measured by radioimmunoassay. Compared with the control group, CGRP decreased in each group (P <0.05 ~ 0.01); CGRP decreased most obviously in LPS group, the lowest point was 6h, the lowest point in Gln and Dex group was delayed to 24h, Gln decreased the lightest , But there was no significant difference between the lowest point of the three groups, P> 0.05.2. In the early stage of endotoxemia, the Dex group showed the slowest decline, but in the recovery period, the Gln group recovered fastest, which was significantly higher than LPS group Still significantly lower than 0h control group, P <0.05. Conclusions Gln and Dex can inhibit the decrease of CGRP. Dex can inhibit the decrease of early CGRP, while Gln can promote the recovery more obviously.