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目的：研究去甲斑蝥素（NCTD）体内对脑胶质瘤G422细胞的抑制作用。方法：建立G422细胞小鼠移植瘤模型，将荷瘤小鼠随机分为模型组、CTX阳性对照组及NCTD高、中、低剂量组（100、50、25 mg·kg-1），每组10只，另设10只为正常对照组，正常对照组每日给生理盐水灌胃，CTX和NCTD组采用腹腔注射方式给药，10 d后双抗体夹心ABC-ELISA法测sIL-2R的含量；酶联免疫ELISA法测IFN-γ含量；剥瘤，计算抑瘤率；剥瘤的同时取脾脏，计算脾脏指数；MTT法检测NCTD对荷瘤小鼠脾脏T、B淋巴细胞增殖的影响。结果：NCTD可降低荷瘤小鼠血清sIL-2R含量，提高IFN-γ含量，抑制G422细胞小鼠移植瘤的生长；对脾脏指数影响较少，可促进荷瘤小鼠的T、B淋巴细胞增殖。结论：NCTD体内外可抑制G422细胞生长，并具有免疫调节作用。“,” Objective: To study the inhibition of NCTD ongliomas in vivo. Methods: The mice with G422 cell xenografts were randomly divided into model group, positive control group, NCTD higher, middle and lower doses groups (100, 50 and 25 mg·kg-1), 10 rats in each group. There is normal control group of 10 rats without inoculated tumour. After 10-day treatment, the serum content of sIL-2R was determined by enzyme-linked immunosorbent assay. The serum content of IFN-γ was detected by ELISA. Tumor was stripped and the tumor-inhibition rate was counted. The spleen was removed, with the spleen index calcu-lated; MTT was performed to observe the effect of NCTD on proliferation of lymphocyte cell T and B. Results: NCTD signifi-cantly decreased the content of sIL-2R in mice, increased the content of IFN-γ, inhibited the growth of G422 cell xenografts, with no significant effect on spleen index and promoted the proliferation of lymphocyte T and B in tumor-bearing mice spleen. Conclusion: NCTD can inhibit the growth of G422 in vivo and promote immunomodulatory effects.