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【目的】通过对玉米幼胚培养,建立～套稳定性好、再生率高的组织培养体系。【方法】以4个玉米自交系幼胚为外植体,研究不同基因型、幼胚大小、2,4-D浓度、AgNO_3浓度对愈伤组织诱导的影响,进一步研究6-BA和蔗糖浓度对愈伤组织分化及IBA和NAA对再生苗的生根影响。【结果】2,4-D浓度为2.0～3.0mg/L,幼胚长度为1.0～2.0 mm时,对胚性愈伤组织诱导有良好效果;在诱导期间采用隔代法添加10 mg/LAgNO_3能提高胚性愈伤组织的诱导率;当6-BA为0.5 mg/L、蔗糖浓度为50g/L时,愈伤组织分化率最高且有利于小植株的形成;IBA浓度为0.6 mg/L对植株生根较有利。【结论】对4个玉米自交系幼胚培养研究筛选出适宜玉米幼胚培养的最佳诱导、分化及生根培养基。
【Objective】 To establish a tissue culture system with good stability and high regeneration rate through maize immature embryo culture. 【Method】 Four maize inbred lines were used as explants to study the effects of different genotypes, immature embryo size, 2,4-D concentration and AgNO 3 concentration on the induction of callus. Further studies on the effects of 6-BA and sucrose Effect of Different Concentrations on Callus Differentiation and Rooting of Regenerated Seedlings with IBA and NAA. 【Result】 The concentration of 2,4-D was 2.0-3.0 mg / L, and the length of embryo was 1.0-2.0 mm, the effect of embryogenic callus induction was good. During the induction period, 10 mg / LAgNO 3 The induction rate of embryogenic callus was increased. When the concentration of 6-BA was 0.5 mg / L and the concentration of sucrose was 50 g / L, the differentiation rate of callus was the highest and the plantlet formation was beneficial. The IBA concentration was 0.6 mg / L Rooting plants on the more favorable. 【Conclusion】 The optimum induction, differentiation and rooting medium for immature embryo culture of four maize inbred lines were screened for immature embryo culture.