论文部分内容阅读
目的:用生化及病理学方法研究叶黄素对二甲基甲酰胺(DMF)所致小鼠急性肝损伤的保护作用。方法:将48只小鼠随机分为4组,分别为对照组、DMF模型组、20mg/kg及40mg/kg叶黄素处理组。小鼠连续灌胃叶黄素5天,对照组和DMF模型组给予等体积玉米油。灌胃叶黄素3天后,除对照组腹腔注射生理盐水,其余三组均一次性腹腔注射1.5g/kg DMF。DMF染毒48h后,所有动物内眦取血,处死后剖取肝脏,检测其AST、ALT等指标。结果:DMF模型组小鼠血清ALT和AST活性显著升高,肝脏组织匀浆中MDA含量明显增加,SOD活性升高。叶黄素可降低血清AST及ALT活性及肝组织SOD活性、MDA水平,改善肝脏损伤小鼠病理组织学变化。结论:叶黄素通过抗氧化作用对DMF所致肝脏毒性具有一定保护作用。
Objective: To study the protective effect of lutein on acute liver injury induced by dimethylformamide (DMF) in mice by biochemical and pathological methods. Methods: 48 mice were randomly divided into 4 groups: control group, DMF model group, 20mg / kg and 40mg / kg lutein group. Mice were fed with lutein continuously for 5 days. The control group and DMF model group were given equal volume of corn oil. After three days of intragastric administration of lutein, except the control group, intraperitoneal injection of saline, and the remaining three groups were intraperitoneal injection of 1.5g / kg DMF. After exposure to DMF for 48h, all the animals were sacrificed and the liver was taken after sacrifice. The AST, ALT and other indexes were measured. Results: The serum ALT and AST activities in DMF model group were significantly increased. The content of MDA in liver tissue homogenate increased significantly and SOD activity increased. Lutein can reduce serum AST and ALT activity and liver SOD activity, MDA levels, improve liver injury in mice histopathological changes. Conclusion: Lutein has a protective effect on hepatic toxicity induced by DMF through anti-oxidation.