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目的探讨丹皮酚对烫伤诱导小鼠肝损伤肝组织自噬蛋白LC3表达的影响。方法小鼠随机分为空白组、模型组和丹皮酚组,用90℃热水建立烫伤模型,丹皮酚组小鼠在建模前1 h用丹皮酚溶液(60 mg·kg-1)灌胃。观察建模后第2,6,12,24 h小鼠血清丙氨酸氨基转移酶(ALT)与血清天门冬氨酸氨基转移酶(AST)水平变化及肝组织LC3表达情况。结果丹皮酚可有效降低小鼠血清ALT、AST水平,与模型组比较差异有统计学意义,同时可以使肝组织LC3表达增加(均P<0.05)。结论丹皮酚通过增加自噬蛋白LC3表达对深Ⅱ度烫伤诱导的肝损伤具有保护作用。
Objective To investigate the effect of paeonol on the expression of autophagy protein LC3 in hepatic tissues induced by scald injury in mice. Methods The mice were randomly divided into blank group, model group and paeonol group, scald model was established by hot water at 90 ℃, paeonol group was treated with paeonol solution (60 mg · kg -1 ) Gavage. The changes of serum ALT and AST and the expression of LC3 in liver tissue were observed at the 2nd, 6th, 12th and 24th hour after modeling. Results Paeonol could effectively reduce the level of serum ALT and AST in mice, which was significantly different from the model group (P <0.05). Conclusion Paeonol can protect LC-induced liver injury by increasing the expression of autophagy protein LC3.