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目的有效控制规模化蛋鸡生产过程中产生的大量氨气。方法通过富集、筛选和验证试验从土壤样本中筛选抑氨菌,通过纸片法将其对从鸡粪中富集到的产氨菌进行了拮抗试验,并进行抑氨菌的尿酸氮和氨氮的优先利用试验。结果成功获得了一株高效抑氨菌CA26,其在3 d内对氨氮的去除率稳定在40%～60%,且对17株产氨菌有拮抗作用,在尿酸氮和氨氮同时存在时优先利用氨氮作为氮源。结论菌株CA26能高效抑制鸡粪氨挥发,其抑氨机制包括对产氨菌的拮抗作用和对氨氮的吸收利用。
Objective To effectively control large-scale production of ammonia in large-scale laying hens. Methods The enrichment, screening and validation tests were carried out to screen for ammonia-inhibitory bacteria from soil samples. Antagonistic tests were carried out on the ammonia-producing bacteria enriched from chicken manure by the paper method. The uric acid nitrogen and Preferential use of ammonia nitrogen test. Results A highly effective ammonia-tolerant bacterium CA26 was successfully obtained. Its ammonia nitrogen removal rate was stable at 40-60% within 3 days and had antagonism against 17 ammonia-producing bacteria. It was given priority when both uric acid nitrogen and ammonia nitrogen coexisted Use ammonia nitrogen as nitrogen source. Conclusion The strain CA26 can effectively inhibit the ammonia volatilization of chicken manure. The ammonia inhibition mechanism includes the antagonism of ammonia-producing bacteria and the absorption and utilization of ammonia nitrogen.