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采用细菌基因组重复序列PCR技术(简称repPCR)中常用的REPPCR和ERICPCR,对从中国11个省、市的23个点、24个花生品种采集的根瘤中分离的59株花生根瘤菌Bradyrhizobiumsp.(Arachis)进行多样性研究,同时对来自国外的6株花生根瘤菌及14株参比慢生根瘤菌也进行了比较。得到的低相似性结果表明中国花生根瘤菌基因组存在显著的多样性。REPPCR揭示,在相似性50%上分为11个群,而ERICPCR却得到24个分群。这两种结果对菌株的分群有差异,暗示这两种短重复序列在慢生根瘤菌基因组中的分布的不同。没有发现菌株间基因组的多样性分布与花生品种、地理来源之间的必然联系。将两者电泳图谱结合并分析,得到介于上述两者间的结果。此结果进一步反映了菌株基因组间存在的多样性。同时还表明repPCR不仅是研究生物多样性的快速简便方法,还可应用于菌株的鉴别和生态学研究。
By using REPPCR and ERICPCR, which are commonly used in bacterial genome repeat PCR (repPCR), 59 peanuts isolated from nodules collected from 23 peanut varieties at 23 sites in 11 provinces and cities in China Rhizobium Bradyrhizobium sp. (Arachis), and also compared 6 peanut rhizobia and 14 reference Bradyrhizobium strains from abroad. The low similarity results show that there is significant diversity in the Chinese peanut rhizobia genome. REP-PCR revealed that in the similarity of 50% is divided into 11 groups, and ERIC-PCR has been 24 sub-groups. The difference between the two results in the grouping of strains suggests that the two short repeats differ in their distribution in the genome of Bradyrhizobium. No association was found between the diversity of bacterial genomes and the geographical origin of peanut varieties. The two electrophoresis patterns were combined and analyzed to get the result between the two. This result further reflects the diversity existing in the strains genome. At the same time, it also shows that rep-PCR is not only a quick and easy method for studying biodiversity, but also can be applied to the identification and ecology research of strains.