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聚甲基戊烯(PMP)膜式氧合器表面先用水等离子体改性,以引入羟基官能团;再以溴化氰为偶联剂,将碳酸酐酶(CA)偶联至其表面。改性后用X射线光电子能谱(XPS)、表面接触角及酶活性测定等方法研究PMP表面性能变化。结果表明,等离子体处理后,在PMP表面引入了大量的含氧官能团,与水的表面接触角从103.37°降低至50.01°。再将CA引入PMP表面后,与水的表面接触角进一步降低至39.23°;XPS的C1谱图中出现蛋白质的特征峰;以对硝基苯酚乙酸酯为底物,测得表面接枝CA的活性达到理论单分子层接枝活性的73%。改性后PMP表面物理化学性质的变化证明本文方法确实能成功地将羟基官能团、CA引入其表面。本方法有望应用在膜式氧合器上以提高其清除血液中CO2的能力。
Polymethylpentene (PMP) membrane oxygenator surface was first modified with water plasma to introduce hydroxyl functional groups; and cyanogen bromide as a coupling agent, coupling carbonic anhydrase (CA) to its surface. After modification, the surface properties of PMP were investigated by X-ray photoelectron spectroscopy (XPS), surface contact angle and enzyme activity determination. The results showed that after plasma treatment, a large number of oxygen - containing functional groups were introduced on the surface of PMP. The contact angle with water decreased from 103.37 ° to 50.01 °. After the introduction of CA into the surface of PMP, the contact angle with water further decreased to 39.23 °. The characteristic peak of protein appeared in the spectrum of C1 of XPS. The surface grafted CA was measured with p-nitrophenol acetate Reached 73% of the theoretical monolayer graft activity. The physical and chemical properties of modified PMP surface proved that the method of this paper can successfully introduce hydroxyl functional group, CA onto its surface. This method is expected to be applied to membrane oxygenator to improve its ability to clear the blood of CO2.